Is HER2 amplification predictable by digital immunohistochemistry?
نویسندگان
چکیده
منابع مشابه
Is HER2 amplification predictable by digital immunohistochemistry?
Background In the last decade anti-HER2 treatment became one of the best examples for targeted treatment. Since the aggressive behavior of HER2-positive breast cancers could have been successfully reduced by trastuzumab therapy, HER2 positive breast cancers recently show improving prognosis. According to a four-tiered classification of international clinical guidelines, cases with strong and co...
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PURPOSE Digital PCR is a highly accurate method of determining DNA concentration. We adapted digital PCR to determine the presence of oncogenic amplification through noninvasive analysis of circulating free plasma DNA and exemplify this approach by developing a plasma DNA digital PCR assay for HER2 copy number. EXPERIMENTAL DESIGN The reference gene for copy number assessment was assessed exp...
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Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2:EFTDU2 ratio by digital PCR assay in the microdissected...
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INTRODUCTION HER2 status assessment became a mandatory test assay in breast cancer, giving prognostic and predictive information including eligibility for adjuvant anti-HER2 therapy. Precise and reliable assessment of HER2 status is therefore of utmost importance. In this study we analyzed breast cancer samples by a novel technology for concomitant detection of the HER2 protein and gene copy nu...
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ژورنال
عنوان ژورنال: Diagnostic Pathology
سال: 2013
ISSN: 1746-1596
DOI: 10.1186/1746-1596-8-s1-s14